- Aquest esdeveniment ja ha passat.
febrer 10 @ 12:00 - 14:00
Understanding CRISPR-Cas dynamics and its allosteric mechanisms by cryo-EM
Speaker: Guillermo Montoya, Prof., Dr. – Research Director, Protein Structure and Function Programme – Novo Nordisk Foundation Center for Protein Research – EMBO Member – Distinguished Investigator Novo Nordisk Foundation – Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.
Genome editing is a type of genetic engineering where a DNA is inserted, deleted, or replaced in the genome of a living organism generating a double-strand break (DSB) in a desired site. To achieve this precise DSB researchers have developed engineered nucleases also termed “molecular scissors”. However, the engineering of new DNA specificities in protein scaffolds is cumbersome. Therefore, the development of the versatile CRISPR-Cas systems (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated proteins) as molecular scissors, where a simple exchange of the RNA guide sequence is enough to redesign the nuclease specificity has paved the way for a revolution in the life sciences. Allostery is a fundamental property in the mechanism of these RNA-guided nucleases, which regulates the precise generation of the DSB that initiates editing. In this talk, I´ll walk you through the new advances in cryo-electron microscopy that permit the visualisation of protein dynamics and how its combination with single-molecule approaches can render key information for protein redesign with important biomedical and/or biotechnological applications.