{"id":50293,"date":"2017-05-03T00:00:00","date_gmt":"2017-05-02T23:00:00","guid":{"rendered":"http:\/\/www.pcb.ub.edu\/a-genome-editing-strategy-to-study-cancer-stem-cells-in-human-tumours\/"},"modified":"2017-05-03T00:00:00","modified_gmt":"2017-05-02T23:00:00","slug":"a-genome-editing-strategy-to-study-cancer-stem-cells-in-human-tumours","status":"publish","type":"post","link":"https:\/\/www.pcb.ub.edu\/en\/a-genome-editing-strategy-to-study-cancer-stem-cells-in-human-tumours\/","title":{"rendered":"A genome-editing strategy to study cancer stem cells in human tumours"},"content":{"rendered":"<p>An innovative methodology proposed by researchers with the\u00a0<a href=\"https:\/\/www.irbbarcelona.org\/en\/research\/colorectal-cancer-laboratory\" target=\"_blank\" rel=\"noopener noreferrer\">Colorectal Cancer Laboratory<\/a>\u00a0at IRB Barcelona opens up the possibility to study human tumours by means of tracing genetic modifications. Such an approach had previously been feasible only in animal models and it may have broad applicability for the analysis of cancer cell diversity during human tumour development\u00a0in vitro\u00a0or after transplantation of tumours to a recipient animal.<\/p>\n<p>\u201cThere is a large interest to study how distinct tumour cell population contribute to the evolution of the disease. Until know, these studies have necessary involved experiments of tumour dissociation and cell isolation, which have raised many conceptual and technical caveats. In essence, this new approach brings the power of mouse genetic tools to study human cancer and overcomes the limitations of identifying and tracking tumour cell populations in an intact environment,\u201d\u00a0explains ICREA Research Professor\u00a0Eduard Batlle,\u00a0who led the study published today in\u00a0EMBO Molecular Medicine.<\/p>\n<p>The researchers combine two novel methodologies, namely patient-derived tumour organoids (an\u00a0organoid\u00a0is a miniaturized and simplified version of an organ that is produced in vitro in three dimensions and that shows realistic micro-anatomy), and CRISPR\/Cas9 genome-editing techniques (which allow the introduction of markers into genes of interest). The scientists consider that the combined method proved \u201cparticularly well suited\u201d for\u00a0analysing the diversity of cell populations within cancers\u00a0as it allows the labelling and tracing of distinct tumour cells through\u00a0desired marker genes.<\/p>\n<p>\u201cFor the first time we can analyse cell lineage relationships in growing tumours, and this technique will help us to address how distinct cell populations contribute to growth, dissemination, and resistance to therapy,\u201d\u00a0wrote the IRB Barcelona authors in reference to the impact expected.<br \/>\n\u00a0<\/p>\n<p><strong>\u2022 For further information:\u00a0<a href=\"https:\/\/www.irbbarcelona.org\/en\/news\/a-genome-editing-strategy-to-study-cancer-stem-cells-in-human-tumours\" target=\"_blank\" rel=\"noopener noreferrer\">IRB Barcelona website\u00a0\u00a0[+]<\/a><\/strong><\/p>\n<p><strong>\u2022\u00a0Reference article :\u00a0<\/strong>Carme Cortina, Gemma Turon, Diana Stork, Xavier Hernando-Momblona, Marta Sevillano, M\u00f2nica Aguilera, S\u00e9bastien Tosi, Anna Merlos-Su\u00e1rez, Camille Stephan-Otto Attolini, Elena Sancho &amp; Eduard Batlle.\u00a0<a href=\"http:\/\/embomolmed.embopress.org\/content\/early\/2017\/05\/02\/emmm.201707550\" target=\"_blank\" rel=\"noopener noreferrer\">A genome editing approach to study cancer stem cells in human tumors<\/a><em>.\u00a0EMBO Molecular Medicine<\/em>\u00a0(2017). DOI:10.15252\/emmm.201707550<\/p>\n<p>\u00a0<\/p>\n","protected":false},"excerpt":{"rendered":"<p>In\u00a0EMBO Molecular Medicine,\u00a0<a href=\"http:\/\/www.pcb.ub.edu\/portal\/en\/cerca?p_p_id=cercador_WAR_empleatsempresesportlet&amp;p_p_lifecycle=0&amp;p_p_state=maximized&amp;p_p_mode=view&amp;p_p_col_id=column-1&amp;p_p_col_count=1&amp;_cercador_WAR_empleatsempresesportlet_jspPage=%2Fhtml%2Fcommon%2Fdetall-empresa.jsp&amp;_cercador_WAR_empleatsempresesportlet_empresaId=ENT_000001&amp;_cercador_WAR_empleatsempresesportlet_redirect=%2Fportal%2Fca%2Fcerca%3Fp_p_id%3Dcercador_WAR_empleatsempresesportlet%26p_p_lifecycle%3D0%26p_p_mode%3Dview%26_cercador_WAR_empleatsempresesportlet_keywords%3Dirb%26_cercador_WAR_empleatsempresesportlet_tab%3D1\" target=\"_blank\" rel=\"noopener noreferrer\">IRB Barcelona<\/a>\u00a0scientists at PCB report a technique based on a combination of CRISPR\/Cas9 genome editing and patient-derived tumour organoids that allows the study of cell heterogeneity in human tumours.The novel approach was used to examine the behaviour of colorectal cancer stem cells by tracing specific marker genes.<\/p>\n<p>\u00a0<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"categories":[8],"tags":[],"class_list":["post-50293","post","type-post","status-publish","format-standard","category-uncategorized"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.7 - 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